Hua Rongo
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Tikanga o Oligo Synthesizer
Te Maataapono o te Oligo Synthesizer I roto i nga mara o te koiora ngota me te rangahau iranga, he mahi nui te kaha ki te whakahiato DNA.Ko te whakahiato DNA ko te hanga horihori o ngota ngota DNA ma te whakarite...Pānuitia atu -
Me pehea te whiriwhiri i te DNA RNA Oligonucleotide Synthesizer
He patai hei patai i mua i te kowhiri i te DNA RNA Oligonucleotide Synthesizer 1. Kei te whakamahi koe i te synthesis mo te R&D, te whakaputa ranei?He rerekee nga reanga taiwhanga e hiahia ana ki nga reanga ture.Ko te tikanga, ka hiahiatia e nga whare whakangao nga tuhinga ture me nga ratonga hei whakarite i te kounga...Pānuitia atu -
Ko nga mea e whakatau ana i te kaha o te whakahiato DNA
I roto i te DNA angamaheni, te RNA me te whakahiato waikawa karihi kore-maori, he mahi nui te taahiraa Deprotection and Coupling.Ko te taahiraa Deprotection ko te tango i te roopu DMT i runga i te tautoko totoka, i te roopu 5' hydroxyl ranei i runga i te nucleoside o mua me te waikawa waro, me te whakaatu...Pānuitia atu -
Te rereketanga i waenga i te DNA me te RNA synthesis
Ko te whakahiato DNA me te RNA kei runga i te rautaki whakahiato-waahanga totoka me te matū phosphoramidite, ka taea te whakamahi i te synthesizer DNA ki te whakahiato i te RNA me te RNA analogues kaore he whakarereketanga, a ka taea te whakamahi tika i nga reagents i roto i te whakahiato DNA i roto i te RNA me te. .Pānuitia atu -
Ko te kaupapa matua o te whakahiato DNA
Ko te whakahiato DNA matū i ahu mai i te rautaki whakahiato-waahanga totoka me te matū phosphoramidite.Rerekē i te kōtuitanga DNA koiora, te rauemi i roto i te kōtuitanga DNA matū ko DMT (4, 4-dimethoxytrityl) me te phosphoramidite deoxyriboxyside whakarerekētia, rite sho...Pānuitia atu
