Ko te whakahiato DNA me te RNA e hangai ana i runga i te rautaki whakahiato-waahanga totoka me te matū phosphoramidite, ka taea te whakamahi i te synthesizer DNA ki te whakahiato i te RNA me te RNA analogues me te kore he whakarereketanga ano, a ka taea te whakamahi tika nga reagents i roto i te whakahiato DNA i roto i te RNA me te waikawa karihi artificial. ' whakahiato.Ka rite ki te Whakaaturanga 1, ka tiakina te 2'-hydroxy i roto i te RNA phosphoramidite ki te roopu tiaki silyl, ara ko t-butyldimethylsilyl (TBDMS), te aukati i nga tauhohenga taha ki te roopu 2'-hydroxy pakarukaru.Ko te roopu TBDMS nui i arai i te tauhohenga i waenga i te phosphoramidite me te roopu 5'-hydroxy i runga i te tautoko totoka, me te roa ake o te wa hono ki te mohio ki te pai o te hono.
He rite ki te whakahiato DNA, i wehea tuatahitia te RNA whakahiato mai i te tautoko totoka e te aminolysis, katahi ka wehea te roopu TBDMS e te tetrabutylammonium fluoride (TBAF) trimethylamine trihydrofluoride ranei.Ka taea te purea te RNA maru ma te whakamaarama mai i te waipiro me te HPLC.
Whakaahua 1. Te hanganga matū o nga poraka hanga taketake i roto i te DNA me te RNA synthesis.
a) dABz phosphoramidite me b) rABz 2'-OTBDMS phosphoramidite.
Ko te whakawhanaketanga o te raau taero siRNA e hiahia ana kia rite ki te hanganga o te RNA taketake hei whakanui ake i te koiora, ko te 2'-hydroxy i runga i te RNA ka taea te whakakapi e te roopu MeO, F me te MOE, me te waikawa nucleic raka (LNA) he pai te mahi i roto i te rongoa RNA. (Whakaahua 2).Ko enei phosphoramidite he rite ki te momo DNA phosphoramidites i roto i te whakahiato, a ko te mahi me te purenga o enei waikawa karihi kore-maori he rite ki te DNA taketake.
Whakaahua 2. Ko te hanganga matū o nga poraka whare i roto i nga raau taero siRNA.a) dABz 2-MeO phosphoramidite;b) dABz 2-F phosphoramidite;c) dABz 2-MOE phosphoramidite me d) dABz Locked phosphoramidite.
Phosphoramideshe mea nui mo te whakahiato ira, tae atu ki te nuinga o nga whanau DNA me te RNA me o raatau huanga, nga phosphoramidite katoa kei runga ake ka taea e matou te tuku i roto i te kete.
Kei te whakapai tonu matou i nga mahi o a matou hua, te whakaoti i a matou mahi whakaputa me te tika o nga korero.Kaore matou i te whakarato noa ki a koe i nga hua o te kounga teitei, engari ka whakawhiwhia ano koe ki te whakangungu me te ratonga.Kua rite matou ki nga kaimahi whakahaere ngaio me nga roopu hangarau tino pai me nga kaimahi tiaki ki te whakarato ki o taatau kaihoko he ratonga tino pai i muri i te hoko.
Wā tuku: Akuhata-09-2022