HY 192 DNA RNA oligo synthesizer mō te tere whakaputa nui
Whakatakotoranga
| Tauine whakahiato hongere kotahi | 5noml-5umol. |
| Te wā huringa whakahiato | tae atu ki te 6-8 meneti |
| Huringa hanga (20mer) | 2-3 hāora |
| Turanga pounamu Phosphoramidite | 8 ngā huinga |
| Papa pounamu tauhohenga | 7 ngā huinga |
| Pounamu turanga | Atanga pounamu matū hohenga whānui 60/240/480ml waha tīwiri |
| Pounamu āwhina | Waha pounamu GL38, whakamahia mō ngā pounamu matū 4L whānui. |
| Tikanga taraiwa tauhohenga | momo hau tiaki i raro i te pēhanga |
| Te tukunga wai para | pēhanga pai |
| Te tere hononga | 99% |
| Te roa mōrahi | neke atu i te 120mer |
| Pūtake hiko | Kotahi-wāhanga 220V. |
| Te pāmahana mahi | 20°± 5°C |
| Makuku whanaunga | i roto i te 40%. |
| Te pumau o te mahi | Ka taea e ia te mahi noa, me te mahi tonu. |
| Aroturuki | LCD |
| Pūtāhui | 1 Tau |
Āhuatanga
1. He hongere motuhake ia matū mai i te pounamu rokiroki wai ki te pou whakahiato, kāore he hongere kē atu e whiti.
2. Ka tāpirihia te kaiwhakaoho me te Phosphoramidite i ia wā i te tukanga honohono, ā, ka whakaranua ki te pou whakahiato hei whakahaere i te tauhohenga.
3. E rua ngā pereti kei roto, e 192 ngā pou whakahiato me ngā putanga pounamu turanga 12-20.
4. Haunga ngā turanga paerewa e 4 me ngā tauhohenga āwhina hangai, e 8 anō hoki ngā turanga kua whakarerekētia, ka taea te hanga i ngā mea e hiahiatia ana, pērā i te thiomodification, ētahi atu whakarerekētanga fluorescent me ngā probe TAQMAN tapanga-rua, me ētahi atu. Neke atu i te 8 ngā turanga motuhake.
5. Ka taea te hanga me te whakakotahi aunoa i ngā turanga, me te kore e hiahiatia te whakaranu i mua i ngā Phosphoramidites.
6. I te wā katoa o te tukanga hanga, he nui te hau kei roto i te rūma hanga (ka taea te whakarite i te nui o te hau). Ka whakakīia te hau tiaki hei ārai i te hau kei uru ki te kōhao hanga, ā, ka pā ki te kounga o te hanga.
7. Ka taea te utaina ngā tauhohenga me te Phosphoramidite ki roto i ngā pounamu e rua i te wā kotahi, ka taea ai te whakanui ake i te wā o te hanganga kotahi me te kore e whakakapia pinepine ngā tauhohenga.
8. He takirere pana horoi, ā, ka taea te whakamahi i te acetonitrile me te argon hei horoi i ngā paipa me ngā takirere hei karo i te aukati i ngā paipa me ngā takirere i puta mai i te noho mangere roa o ngā taputapu.
9. Kei ngā taputapu ngā mahi tirotiro-ā-koe me te tiaki, whakaweto, whakamutu me te haere tonu.
10. Ko ngā tikanga mahi me ngā whakaritenga haumaru e tutuki ana ki ngā paerewa me ngā ture Hainamana me te ao whānui.
