Taputapu purenga mo te purenga Oligo
Whakatakotoranga
| Papa hototahi | 1, 3, 5, 8. |
| Tātari | pupuhi filtration, momi filtration |
| Te maha o nga tauranga werohanga | 5, 6, 7, 8, 9, 10. |
| Nga momo pereti hototahi | Pereti C18, pereti puna hohonu, pereti waihanga (hototahi ki te nuinga o nga pereti waihanga), pereti microtiter |
| Kōwae | tuaka kotahi, tuaka rua ranei |
| Ngaohiko | 220V |
| Pūtāhui | 1 tau |
| Ritenga | Whakaaetia |
Nga huarahi rereke o te Pure
1. Gel Electrophoresis Chromatography Purification
Whakamahia te polyacrylamide gel electrophoresis chromatography mo te purenga.Ko te tikanga he 4M formamide, he 7M urea ranei te kaikawe denaturing.
I muri i te electrophoresis, me whakatau te tuunga o te roopu waikawa karihi i raro i te whakamaaramatanga o te rama ultraviolet, ka tapahia te kiri kei roto te waikawa karihi whaainga, ka pakaruhia te waikawa karihi ka rewa, katahi ka kukū te wairewa riihi, ka whakahekehia, te ine me te lyophilized.
2. DMT-On, HPLC Purification
Whiriwhiria te aratau DMT-On i te wa o te whakahiatotanga, ko te hua masivesivá i centrifuged me te kukū ki te pāmahana rūma ki te tango haukini i te taikaha i muri i te aminolysis.
I mahia te wehenga ma te whakamahi i te pou C18 me te acetonitrile me te 10% triethylamine-acetic acid (TEAA) hei eluent.I muri i te otinga o te elution, ka arohia, katahi ka tangohia te roopu DMT me te waikawa trifluoroacetic.Whai muri i te whakakorenga, ka tangohia etahi tai me etahi ngota ngota iti ma te ngongo kua tapahia, ka mutu ka whakakorehia.
Ka taea e tenei tikanga te tiki i tetahi hua me te maamaa teitei ake, engari he mea tika kia aro ki te puta o te whakahekenga.
3. DMT-Off, HPLC Purification
Whiriwhiria te DMT-Off i te wa o te whakahiatotanga, ka whakahiatotia te hua masivesivá, ka whakakotahitia ki te pāmahana rūma hei tango i te haukini i te taikaha i muri i te ammonolysis.
I whakahaerehia te wehenga ma te whakamahi i te pou C18 me te acetonitrile me te 10% te waikawa triethylamine-acetic i roto i te wai hei eluent.I muri i te otinga o te wehenga me te ine, ka lyophilized nga aliquot.
Ko tenei tikanga me ata whakatikatika nga tikanga wehenga, ka taea hoki te whiwhi i nga ngota ngota tino parakore.
