Ko nga taputapu purenga Oligo mo te whakahiato tuatahi o Oligo

Ko nga taputapu horoi Oligo mo te whakahiato tuatahi o Oligo,
miihini tautoko whakahiato oligo,

Whakatakotoranga

Nga huarahi rereke o te Pure

1. Gel Electrophoresis Chromatography Purification
Whakamahia te polyacrylamide gel electrophoresis chromatography mo te purenga.Ko te tikanga he 4M formamide, he 7M urea ranei te kaikawe denaturing.
I muri i te electrophoresis, me whakatau te tuunga o te roopu waikawa karihi i raro i te whakamaaramatanga o te rama ultraviolet, ka tapahia te kiri kei roto te waikawa karihi whaainga, ka pakaruhia te waikawa karihi ka rewa, katahi ka kukū te wairewa riihi, ka whakahekehia, te ine me te lyophilized.

2. DMT-On, HPLC Purification
Whiriwhiria te aratau DMT-On i te wa o te whakahiatotanga, ko te hua masivesivá i centrifuged me te kukū ki te pāmahana rūma ki te tango haukini i te taikaha i muri i te aminolysis.
I mahia te wehenga ma te whakamahi i te pou C18 me te acetonitrile me te 10% triethylamine-acetic acid (TEAA) hei eluent.I muri i te otinga o te elution, ka arohia, katahi ka tangohia te roopu DMT me te waikawa trifluoroacetic.Whai muri i te whakakorenga, ka tangohia etahi tai me etahi ngota ngota iti ma te ngongo kua tapahia, ka mutu ka whakakorehia.

Ka taea e tenei tikanga te tiki i tetahi hua me te maamaa teitei ake, engari he mea tika kia aro ki te puta o te whakahekenga.

3. DMT-Off, HPLC Purification
Whiriwhiria te DMT-Off i te wa o te whakahiatotanga, ka whakahiatotia te hua masivesivá, ka whakakotahitia ki te pāmahana rūma hei tango i te haukini i te taikaha i muri i te ammonolysis.
I whakahaerehia te wehenga ma te whakamahi i te pou C18 me te acetonitrile me te 10% te waikawa triethylamine-acetic i roto i te wai hei eluent.I muri i te otinga o te wehenga me te ine, ka lyophilized nga aliquot.

Ko tenei tikanga me ata whakatikatika nga tikanga wehenga, ka taea hoki te whiwhi i nga ngota ngota tino parakore.

Ko nga taputapu accerry oligo synthesis mo te huringa kōtuitanga dna rna, e pai ana mo te tuatahi o te waa, me etahi atu waahanga mekameka poto, ka tino ma te oligo.
te kaihanga o Haina o nga taputapu oligo synthesis me te kounga pai me te ratonga pai.