Ko nga mea e whakatau ana i te kaha o te whakahiato DNA

I roto i te DNA angamaheni, te RNA me te whakahiato waikawa karihi kore-maori, he mahi nui te taahiraa Deprotection and Coupling.

Ko te taahiraa Deprotection he tango i te roopu DMT i runga i te tautoko totoka, i te roopu 5' hydroxyl ranei i runga i te nucleoside o mua me te waikawa waro, me te whakaatu i te roopu hydroxyl mo te hikoi hono e whai ake nei.Ko te waikawa trichloroacetic 3% kei roto i te dichloromethane, i te toluene ranei te nuinga o te whakamahi ki te kawe i te taahiraa whakamarumaru.Ko te kukū o te waikawa trichloroacetic me te wa whakamarumaru (te wa whakakorea) te rangatira o te ma o nga hua whakamutunga.Ko te iti o te kukū me te iti o te wa wetewete ka waiho he roopu DMT kaore i aro, ka heke te hua me te whakanui ake i nga poke kore.Ko te roa o te wa wetewete ka arahi i te depurine o nga raupapa whakahiato, ka puta nga poke ohorere.

He tairongo te takahanga hono ki te wai o nga whakarewa me te makuku o te hau.Ko te kukū o te wai i roto i te whakahiato me iti iho i te 40 ppm, pai ake i te 25 ppm.Hei pupuri i te ahua o te whakahiatotanga anhydrous, me whakahaere te whakahiatotanga o nga waikawa nucleic i roto i te taiao haumākū iti, na reira ka tūtohu matou ki ta taatau kaihoko ki te whakamahi i teAmidites Taputapu Whakarewa, ka taea te whakakore i te Phosphoramidite paura, hinuhinu ranei i roto i te acetonitrile anhydrous kia kore ai e pa ki te hau.