Nga mahanga ngota mo te phosphoramidite me nga reagents
Whakaahuatanga Hua
I roto i nga whakamatautau ABI me te MilliGen/PerSeptive DNA synthesis, ka taea e te paatete iti te pupuri i te ihirangi wai i roto i te nitrile me nga ipu whakaoho i raro iho i te 10 ppm, me te kore e awangawanga mo te riipene, te riipene taraiwa ranei kua wehea ki roto i te tatari.Ko te puehu, te kirikiri ranei kei runga i te peeke takai kua purua.Ka taea te whakamahi tika mo te whakamaoritanga o te 500 ml, 1L, 2 L me etahi atu ipu whakarewa, a ka taea hoki te whakarite i nga korero mo te putea iti.
Ko nga mea e whai ake nei e whakaatu ana i te kaha whakaheke wai o te 10 g mahanga ngota i roto i nga tauira 500ml, 1L, 4L nitrile me nga momo wai rereke.
Whakatauritea ki te mahanga irapoi kawemai
| 500 ml 197 ppm ACN | |||||
| Wā(h) | 0 | 24 | 48 | 72 | 96 |
| HonyaBio | 197 | 33 | 16.5 | 6.5 | 6 |
| Ētahi atu Whenua | 197 | 43 | 27 | 15 | 15 |
| 1 L 143 ppm ACN | |||||
| Wā(h) | 0 | 24 | 48 | 72 | 96 |
| HonyaBio | 143 | 48 | 32 | 20 | 15 |
| Ētahi atu Whenua | 142 | 47 | 36 | 23 | 15 |
| 4 L 141 ppm ACN | |||||
| Wā(h) | 0 | 24 | 48 | 72 | 96 |
| HonyaBio | 141 | 95 | 94 | 84 | 73 |
| Ētahi atu Whenua | 141 | 96 | 95 | 85 | 72 |
Nga tohutohu me te whakamahi
Ko te mahanga irapoi he mahanga-kore, a me whakatuwherahia ahakoa ka whakamahia, me te hiri o te pounamu reagent me whakarite i te wa e whakamahia ana.
Ka taea e te tatari 2g mo te 24 haora te whakaheke i te 165 ppm wai i roto i te 500 ml nitrile ki te 105 ppm.
Ko te 5 g tatari mo te 24 haora ka taea te whakaheke i te 172 ppm wai i roto i te 500 ml nitrile ki te 58 ppm.
10 g tatari 24 h Ka taea e ia te whakaheke i te 166 ppm wai i roto i te 1 L nitrile ki te 68 ppm.
Ka taea e te 20 g iti tatari te whakaheke i te 162 ppm te wai i roto i te 4 L nitrile ki te 109 ppm mo te 24 haora.
Ka tūtohu matou 2 g subsieve mo 50-250ml ipu reagent, 5g mo 250-500ml ipu reagent, 10g mo 500-1000ml ipu reagent, me te 20g mo 1000-2000ml ipu reagent.
Whakapumautanga
Ko ia kete iti-mata ka mau te mana kounga i te wa o te mahi whakaputa.A he mea tika kia tirohia e nga kaihoko te kohinga i mua i te whakamahi:
Tuatahi, whakaū kei te mau tonu te putunga putunga.Ko nga rerenga korehau, te urunga hau ranei ka iti te mahi o te hua.
Tuarua, kia tupato i te wa e whakatuwhera ana i te putunga kia kore ai e rakuraku te kiriata takai-iti, ka tirohia mena he turuturu te kiriata.
Te maimoatanga i muri i te whakamahinga
Ehara i te mea paitini, i te kino ranei, ka pangia e nga matū me nga whakarewa ka pa atu ki a raatau, me waiho hei para poke i muri i te whakamahinga.
Tauira me te tono
Kua whakaratohia e matou i mua i nga putea iti i roto i te rahi o te 2 g, 5 g, 10 g, me te 20 g., me etahi atu waahanga ka taea te whakarite kia rite ki nga hiahia o nga kaihoko.
Ko te mahanga ngota he pai mo te wa roa e pa ana ki te nitrile, ka taea hoki te whakamahi mo te waikawa acetic, ether, acetic ether, butyl acetate, waipiro, isopropanol, methanol, butanol, Phenol, pyridine, hydrochloric acid, nitric acid, phosphoric acid. , waikawa sulfuric, methyl chloride, hauota methyl imidazole, etc.
Kaore pea e pai mo te wa roa ki te tetrahydrofuran, toluene, methyl formamide (DMF), methyl methyl amide (DMAc), N-methylpyrrolidone (NMP) me etahi atu otinga.
